hCG+ b, IRMA

The DiaSource hCG+β - IRMA is an immunoradiometric assay based on coated tube separation in which several monoclonal antibodies directed against distinct epitopes of hCG have been used.

Mabs 1 - the capture antibodies are attached to the lower and inner surface of the plastic tube. Calibrators or samples added to the tubes will at first show low affinity for Mabs 1.

Addition of Mabs 2, the signal antibody labelled with 125I, will complete the system and trigger the immunological reaction. After washing, the remaining radioactivity bound to the tube reflects the antigen concentration