hGH, IRMA
The DIAsource hGH-IRMA is an immunoradiometric assay based on coated-tube separation.
Mab 1 - the capture antibody is attached to the lower and inner surface of the plastic tube.
Calibrators or samples added to the tubes will at first show low affinity for Mab 1.
Addition of Mab 2, the signal antibody labeled with 125I, will complete the system and trigger the immunological reaction.
After washing, the remaining radioactivity bound to the tube reflects the antigen concentration