Somatostatin, RIA, (RUO) Research Use Only

The intended use is for determination of somatostatin in human plasma.

Somatostatin in plasma is extracted with Sep-pak C18 cartridges. The extracts are analysed by a competitive radioimmunoassay using an antiserum to synthetic cyclic somatostatin 14.

Somatostatin in standards and samples compete with 125I-labelled somatostatin in binding to the antibodies. 125I-Tyr1-somatostatin binds in a reverse proportion to the concentration of somatostatin in standards and samples. Antibody-bound 125I-Tyr1-somatostatin is separated from the unbound fraction using the double antibody solid phase precipitation technique.

The radioactivity of the precipitates is measured.