Glucagon in plasma is assayed by the competitive radioimmunoassay using a rabbit antiserum raised against a glucagon-albumin conjugate.
Glucagon in standards and samples compete with 125I-labelled glucagon in binding to the antibodies in a two steps incubation. 125I -glucagon binds in a reverse proportion to the concentration of glucagon in standards and samples . Antibody-bound 125I -glucagon is separated from the unbound fraction using double antibody solid phase. The radioactivity of the bound fraction is measured in a gamma counter.
The antiserum used in this assay shows less than 0.1% cross reaction with gut-GLI (14). For professional use within a laboratory.