The present method is based on a competitive radioimmunoassay (RIA).

During the incubation, the monoclonal anti-Tg antibody on solid phase competes with the sample/calibrator anti-Tg autoantibodies for the specific sites of the Iodine125 labelled Tg (tracer). After aspiration and washing, the radioactivity in the tubes is measured in a gamma counter. The degree of binding will be inversely proportional to the sample anti-Tg autoantibody concentration.

The calibrators of RIAZENco TG Ab are calibrated against the TgAb international reference preparation MRC65/93.

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