The present method is based on a competitive radioimmunoassay (RIA).
During the incubation, the sample/calibrator aldosterone competes with the aldosterone labeled with Iodine 125 (tracer) for the specific sites of the antiserum coated on the tubes. Separation is based on the use of antibody coated tubes, where the anti-aldosterone antiserum is fixed on the tube walls. After aspiration, the radioactivity in the tubes is measured in a gamma counter.
The degree of binding will be inversely proportional to the sample/calibrator hormone concentration.
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