Rubella (RUB) IgG, ELISA

The kit is intended for detection of specific IgG antibodies in a sample by means of a sandwich type of the ELISA method (i.e. a solid phase coated with specific antigen – antibody from the analysed sample – labelled antibody).

The labelled antibody (conjugate) is an animal immunoglobulin fraction to human IgG conjugated with horseradish peroxidase.

Peroxidase activity is determined in the test by a substrate containing TMB. Positivity is indicated when blue colour appears; after stopping solution has been added, blue changes to yellow. The yellow colour intensity is measured by a photometer at 450 nm, and it is proportional to the concentration of specific IgG antibodies in the sample.