The kit is intended for the detection of anti-treponemal IgM antibodies in a sample by means of a capture type of the EIA method (i.e. a solid phase coated with monoclonal antibody to human IgM immunoglobulins – antibody from the analysed sample – specific antigens labelled with peroxidase).
Specific antigens of Treponema pallidum conjugated with horseradish peroxidase are used for the detection of antibodies.
Peroxidase activity is determined in the test by a substrate containing TMB. Positivity is indicated when blue colour appears; after stopping solution has been added, blue changes to yellow. The yellow colour intensity is measured by a photometer at 450 nm, and it is proportional to the concentration of specific antibodies in the sample.