Varicella Zoster IgG, ELISA

The kit is intended for detection of specific IgG antibodies in a sample by means of a sandwich type of the EIA method (i.e. a solid phase coated with specific antigen – antibody from the analysed sample – labelled antibody).

The labelled antibody (conjugate) is an animal immunoglobulin fraction to human IgG conjugated with horseradish peroxidase.

Peroxidase activity is determined in the test by a substrate containing TMB.

Positivity is indicated when blue colour appears; after stopping solution has been added, blue changes to yellow.

The yellow colour intensity is measured by a photometer at 450 nm, and it is proportional to the concentration of specific IgG antibodies in the sample.

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