Pancreatic Polypeptide (PP), RIA

The intended use of these reagents is for assay of PP in human serum. PP in serum is assayed without extraction by a competetive radioimmunoassay using a rabbit antiserum raised against bovine PP. PP in standards and samples compete with 125I-labelled human PP in binding to the antibodies. 125I-PP binds in a reverse proportion to the concentration of PP in standards and samples. Antibody-bound 125I-PP is separated from the unbound fraction using the double antibody-polyethyleneglycol precipitation technique. The radioactivity of the precipitates is measured. Human, synthetic PP is used for standardization

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