CanAg CA 125 - ELISA

The CanAg CA125 EIA kit is intended for the quantitative dertermination of the cancer associated antigen C125 in Serum

CanAg CA125 EIA is a solid-phase test. This non-competitive immunoassay is based on the direct sandwich method.

Calibrators, Controls and Patient samples are incubated together with a biotinylated Anti-CA125 (mouse) monoclonal antibody (Mab) OV 197 in Streptavidin-coated microtiter strips. CA125, present in Calibrators or samples, is absorbed onto Streptavidin-coated strips by biotinylated Anti-CA125 Mab, during incubation. The strips are then washed and incubated with HRP-labelled Anti-CA125 Mab OV 185.

After washing, Chromogenic Substrate Buffered Reagent (Hydrogen Peroxide and 3, 3', 5, 5' tetra-methyl-benzidine) is added to each well and the enzymatic reaction can begin.

During the enzymatic reaction, a blue color develops if antigen is present. The intensity of color development is proportional to the amount of CA125 present in the samples. Color intensity is determined by a microtiter plate spectrophotometer at 620 nm (or optionally at 405 nm) after addition of the Stop Solution.

Calibration curves are constructed for each test by plotting absorbance values against the concentration of each Calibrator. The CA125 concentrations of patient samples are then read from the calibration curve.