The CanAg AFP EIA kit is intended for the quantitative dertermination of α -Fetoprotein (AFP) in human serum.  

CanAg EIA a solid-phase test. This non-competitive immunoassay is based on the direct sandwich technique.

Calibrators, Controls and Patient samples are incubated together with biotinylated Anti-AFP monoclonal antibody and RaIFO/rt Peroxidase (HRP) labeled Anti-AFP monoclonal antibody, in Streptavidin-coated microtiter strips.

After washing, the buffered Substrate / Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetra methyl benzidine) is added to each well and the enzymatic reaction can begin.

During the enzymatic reaction, a blue color develops if Antigen is present. The intensity of the color is proportional to the amount of AFP present in the samples. Color intensity is determined by a microtiter plate spectrophotometer at 620 nm (or optionally at 405 nm after addition of Stop Solution).

Calibration curves are constructed for each assay by plotting absorbance values against the concentration of each Calibrator. The AFP concentrations of the Patient samples are then read from the calibration curve.

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