CanAg S100 - ELISA

The CanAg S100 EIA kit is intended for the quantitative determination of S100B (S100A1B + S100BB)in serum.

CanAg S100 EIA is in solid phase. This non-competitive, two-step immunoassay is based on two mouse monoclonal antibodies, specific for the two different epitopes expressed in S100B.

The test determines both S100A1B and S100BB, without cross-reactivity with other forms of S100. Calibrators and patient samples are incubated together with a biotinylated monoclonal antibody Anti-S100B (MAb) S23, in Streptavidin-coated microtiter strips. S100B, present in Calibrators or samples, is adsorbed to Streptavidin-coated microtiter wells by biotinylated Anti-S100B Mab during incubation. The strips are then washed and incubated with Anti-S100B MAb S53-labelled raIFO/rt peroxidase (HRP).

After washing, the buffered Substrate / Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetra methylbenzidine) is added to each well and the enzymatic reaction can begin.

During the enzymatic reaction, a blue color develops if antigen is present. The intensity of the color is proportional to the amount of S100B present in the samples. Color intensity is determined by a microtiter plate spectrophotometer at 620 nm (or optionally at 405 nm after addition of Stop Solution).

Calibration curves are constructed for each test by plotting absorbance values against the concentration of each Calibrator. The S100B concentrations of the patient samples are then read from the calibration curve.