The DRG Free β-HCG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.
The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on a Free β-hCG molecule.
An aliquot of patient sample containing endogenous Free β-hCG is incubated in the coated well with enzyme conjugate, which is an anti-β-HCG antibody [rabbit] conjugated with horseradish peroxidase.
After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of Free β-HCG in the sample.
Having added the substrate solution, the intensity of colour developed is proportional to the concentration of Free β-HCG in the patient sample.