The DiaSource hPL ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on an hPL molecule.
An aliquot of patient sample containing endogenous hPL is incubated in the coated well with enzyme conjugate, which is a monoclonal anti-hPL antibody conjugated with horseradish peroxidase.
After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of hPL in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of hPL in the patient sample.