PROG, ELISA
The DiaSource Progesterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Progesterone molecule.
Endogenous Progesterone of a patient sample competes with a Progesterone horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Progesterone in the sample.
After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Progesterone in the patient sample.
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