The DiaSource DHEA-S ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with a polyclonal antibody directed towards a unique antigenic site of the DHEA-S molecule.
Endogenous DHEA-S of a patient sample competes with a DHEA-S horseradish peroxidase conjugate for binding to the coated antibody.
After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of DHEA-S in the sample.
After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of DHEA-S in the patient sample.