Androstenedione, ELISA
The DiaSource Androstenedione ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with an antibody directed towards an antigenic site on the Androstenedione molecule. Endogenous Androstenedione of a patient sample competes with an Androstenedione horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase conjugate is reverse proportional to the concentration of Androstenedione in the sample.
After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Androstenedione in the patient sample.
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