IGFBP-1, ELISA

This enzyme immunoassay kit is suited for measuring IGFBP-1 in human serum or Heparin-plasma or in other body fluids, for example amnion fluid, mother milk, urine or saliva, as for diagnostic and scientific purposes.

It is also suited to quantitate IGFBP-1 in cell culture media.

The DiaSource IGFBP-1 Elisa KAPME01 is a so-called Sandwich-Assay.

It utilizes two specific and high affinity antibodies for this protein. The IGFBP-1 in the sample binds to the immobilized first antibody on the microtiter plate.

In the following step, the biotinylated and Streptavidin-Peroxidase conjugated second specific anti-IGFBP-1-Antibody binds in turn to the immobilised IGFBP-1.

Finally, the bound peroxidase catalyses the substrate reaction resulting in a colored product.

Therefore colour intensity is highly specific and quantitatively depending on the IGFBP-1-level of the samples.