IGFBP-2, ELISA
The DIAsource IGFBP-2 KAPME05 Elisa kit is a so-called Sandwich-Assay using two specific and high affinity antibodies.
The IGFBP-2 in the samples binds to the first antibody coated on the microtiter plate.
In the following step the second specific anti-IGFBP-2-Antibody binds in turn to the immobilised IGFBP-2.
The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate.
In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the IGFBP-2-level of the samples.
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