Acid Labile Subunit (ALS), ELISA, (RUO) Research Use Only

The DiaSource ELISA for ALS KAPME35 is a so-called Sandwich-Assay.

It utilizes two specific and high affinity antibodies for this protein. These antibodies were created by immunization of rabbits with specific peptides as previously described by Khosravi and Stadler.

The ALS in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin-Peroxidase conjugated second specific anti-ALS-Antibody binds in turn to the immobilized ALS. In the closing substrate reaction the turn of the colour will be high specific catalysed, quantitatively depending on the ALS-level of the samples.