Cyclosporine, RIA CT
The specific assay of cyclosporine A is a radioimmunological competition assay.
Unknown samples, controls, and calibrators are incubated in monoclonal antibody-coated tubes with 125I‑labeled cyclosporine tracer.
After incubation, the liquid content of tubes is aspirated and bound radioactivity is measured.
A calibration curve is established and unknown values are determined by interpolation from a calibration curve.
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