CgA, RIA

The intended use of these reagents is the determination of Chromogranin A in human serum or plasma.

The basic principle for determination of Chromogranin A with the Chromogranin A RIA kit is the competitive radioimmunoassay using antibodies against human chromogranin A. Chromogranin A in calibrators and samples compete with 125I-labeled chromogranin A in binding to the antibodies. The 125I-chromogranin A binds to the antibodies in an inverse proportion to the concentration of chromogranin A in calibrators and samples. Antibody-bound 125I-chromogranin A is separated from the unbound fraction using the double antibody solid phase technique.

The bound fraction of 125I-chromogranin A is measured in a gamma counter.

 

* This product is also available under RUO labelling. For more information :

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