Radioimmunoassay for the Quantitative Determination of 11- Desoxycortisol (11-DOC) in Human serum.

Since the concentrations of 125I – 11-DOC and coated antibodies are constant, the advancing state of the equation depends on the concentration of 11-DOC.

The amount of 125I – 11-DOC bound to the coated tube is inversely proportional to the concentration of 11 DOC in the sample.

Following the incubation, the tube is aspirated to remove excess unbound 125I labelled 11-DOC.

Patient sample concentrations are read from a calibration curve.

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