Radioimmunoassay for the Quantitative Determination of Estrone in Human plasma or serum.
Since the concentrations of 125I - E1 and coated antibodies are constant, the advancing state of the equation depends on the concentration of E1. The amount of 125I - E1 bound to the coated tube is inversely proportional to the concentration of E1 in the sample.
Following the incubation, the tube is washed to remove excess of unbound 125I - E1. Patient samples concentration are read from a calibration curve.