Radioimmunoassay for the Quantitative Determination of Estrone in Human plasma or serum.

Since the concentrations of 125I - E1 and coated antibodies are constant, the advancing state of the equation depends on the concentration of E1. The amount of 125I - E1 bound to the coated tube is inversely proportional to the concentration of E1 in the sample.

Following the incubation, the tube is washed to remove excess of unbound 125I - E1. Patient samples concentration are read from a calibration curve.

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