Acetylcholine Receptor Autoantibodies, RIA
Acetylcholine receptor (ARAb) from human muscle is used as antigen in this radio receptor assay.
The receptors are labelled with 125I-alpha-bungarotoxin. This snake venom binds the receptors most specifically and almost irreversible. Autoantibodies present in the patient´s serum attach to the labelled receptors.
The resulting immune complexes are precipitated with anti-human IgG. The amount of radioactivity of the sediment is directly proportional to the concentration of acetylcholine receptor autoantibodies of the sample.
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