The kit is intended for detection of specific IgA antibodies to Toxoplasma gondii in a sample by means of a capture type of the ELISA method (i.e. a solid phase coated with animal antibody to human IgA immunoglobulins – antibody from the analysed sample – tracer, i.e. specific antigen with labelled antibody).
The labelled antibody is a mouse monoclonal antibody to p30 surface protein of Toxoplasma gondii conjugated with horseradish peroxidase.
Peroxidase activity is determined in the test by a substrate containing TMB.
Positivity is indicated when blue colour appears; after stopping solution has been added, blue changes to yellow. The yellow colour intensity is measured by a photometer at 450 nm, and it is proportional to the concentration of specific antibodies in the sample.