Toxoplasma (TOXO) IgM, ELISA

The kit is intended for detection of specific IgM antibodies to Toxoplasma gondii in a sample by means of a capture type of the ELISA method (i.e. a solid phase coated with animal antibody to human IgM immunoglobulins – antibody from the analysed sample – tracer, i.e. specific antigen with labelled antibody).

The labelled antibody is a mouse monoclonal antibody to p30 surface protein of Toxoplasma gondii conjugated with horseradish peroxidase.

Peroxidase activity is determined in the test by a substrate containing TMB. Positivity is indicated when blue colour appears; after stopping solution has been added, blue changes to yellow. The yellow colour intensity is measured by a photometer at 450 nm, and it is proportional to the concentration of specific antibodies in the sample.