CanAg Free PSA - ELISA

The CanAg Free PSA EIA is intended for the quantitative dertermination of Free PSA (Prostate Specific Antigen) in human Serum.

The CanAg Free PSA EIA is a non-competitive solid-phase immunoassay based on the direct sandwich technique.

Calibrators, controls and patient samples are incubated together with a biotinylated Anti-PSA Libre monoclonal antibody and raIFO/rt peroxidase (HRP) labeled Anti-PSA monoclonal antibody, in Streptavidin-coated microtiter strips.

After washing, the buffered Substrate/Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetra methylbenzidine) is added to each well and the enzymatic reaction can begin.

During the enzymatic reaction, a blue color develops if antigen is present. The intensity of the color is proportional to the amount of Free PSA present in the samples. Color intensity is determined by a microtiter plate spectrophotometer at 450 nm.