The Angiotensin I RIA serves for the quantitative determination of plasma renin activity (PRA) by the radioimmunoassay of the product of the reaction, Angiotensin I.
The generation of Angiotensin I is the result of the enzymatic cleavage of the renin substrate, angiotensinogen, in plasma samples in the presence of ACE inhibitor (ACE - Angiotensin-Converting Enzyme), an enzymatic inhibitor that blocks the conversion of angiotensin I to angiotensin II.
The immunoassay of Angiotensin I is a radioimmunological competition assay.
Unknown samples, control, and calibrators are incubated in polyclonal antibody-coated tubes with 125I-labeled angiotensin I as tracer. After incubation, the contents of the tubes are aspirated. The bound radioactivity is then determined in a gamma counter.
A standard curve is established and unknown values are determined by interpolation from the standard curve.
* This product is also available under RUO labelling. For more information :