Understanding the widely-used ELISA: A brief overview

Enzyme-Linked Immunosorbent Assay (ELISA) is a widely used laboratory technique for detecting and quantifying substances such as peptides, proteins, antibodies, drugs, and hormones. The technology of ELISA) is performed in a polystyrene plate, typically 96-well plates coated.  

The technique of ELISA operates on the principle of binding enzyme-labelled antigens or antibodies to specific targets, facilitating the detection and quantification of analytes in biological samples with high sensitivity and specificity.

In an ELISA, an antigen is immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a colorimetric signal, which is quantifiable by absorbance.

There are four major types of ELISA:

Direct ELISA 
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Indirect ELISA 
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Sandwich ELISA 
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Competitive ELISA
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Why ELISA Technology?

The ELISA tests stand as a widely respected laboratory method, prized for its sensitivity and specificity in detecting various analytes, from biomarkers to hormones and beyond. Its precision and reliability make it indispensable in biomedical research, clinical diagnostics, and other fields.

ELISA exhibits advantages such as a simple procedure, high efficiency, as simultaneous analyses can be performed without complicated sample pre-treatment. ELISA are cost-effective assay, as low-cost reagents are used.

Who can benefit from ELISA utilization?

ELISA finds utility across a spectrum of sectors, including private or public clinical laboratories, life science research facilities, medical institutions, pharmaceutical companies, biotechnology firms, and academia. Whether you are a researcher, clinician, or industry professional, integrating ELISA technology into your workflow can prove advantageous for your unique requirements.

Numerous entities, spanning institutions and corporations, have experienced firsthand the advantages of ELISA technology. From foundational research endeavors to diagnostic endeavors and drug evaluations, ELISA consistently delivers precise and dependable results.

ELISA can enhance your work, backed by 
DiaSource's support in Belgium and around the Globe

Discover DiaSource's specialized support in the realm of ELISA technology. We recognize ELISA's pivotal role across diverse industries, and at DiaSource, we're committed to guiding you and serving as your dependable ally in fully leveraging its capabilities.

DiaSource fully grasps the significance and intricacies of ELISA technology. Our extensive experience in its application ensures we can provide not just the essential products and services, but also the tailored advice and assistance necessary for your success.

Explore further

If you are keen to explore how ELISA can elevate your projects or operations, reach out to us today. Our expert team stands ready to address your queries and provide the tailored assistance required to maximize the potential of this powerful technology.

Our expertise in ELISA’s

Key points

DiaSource has a respectful image of well-known supplier of ELISA assays with more than 
30 years of experience in IVD

The DiaSource ELISA portfolio covers a wide variety of disease fields from Adrenal function & Hypertension, Autoimmunity, Biogenic Amines & Neurosciences, Bone Metabolism, Cancer Markers, Diabetes & Metabolism, Fertility, Gastrointestinal Metabolism, Growth Factors, Inflammation Markers, Infectious Diseases, Thyroid Function.

DiaSource has a full control of its supply chain, from the production of the raw materials to the aftersales service. The use of these antibodies and matching antigens in DiaSource ELISA immunoassays guarantees a high quality and uncompromising quality control. 

ELISA is a versatile and widely adopted method in clinical diagnostics, research, and industrial applications due to its reliability, ease of use, and adaptability

High sensitivity and specificity: ELISA can detect very low levels of antigens or antibodies with minimal cross-reactivity.

ELISA is safe, easy to handle and relatively cost-effective; its format is suitable for high-throughput screening of large numbers of sample

Quantitative and qualitative: ELISA Can be used for both measuring precise amounts of antigen and simply detecting the presence or absence of the target.

Thanks to our expertise, dedicated and experienced staff, DiaSource provides the required support to all the customers. 

DiaSource offers a complete instrumentation panel for the partial or full automatization of its ELISA assays.