Our expertise in Radioimmunoassay’s
What is RIA technology?
Radioimmunoassay (RIA) is an immunological analysis technique used to measure the concentrations of antigens (such as hormones, drugs, or proteins) in a sample by the use of antibodies. The RIA is based on the competition between a 125Iodine radio-labelled antigen (emitting gamma radiations) and a non-labelled antigen present in a sample to bind to a specific antibody. This competition allows for the measurement of the concentration of the target analyte in a biological sample with high sensitivity and specificity. The bound antigens are then separated from the unbound ones, and the radioactivity of the bound antigen is measured using a gamma counter.
Beside the RIA, the sandwich IRMA technique uses two antibodies, both specific for the same antigen, but binding to two different epitopes. One of the antibodies is coated on to the solid phase. A second antibody labelled with 125I is added to the solid phase. This labelled antibody binds with the antigen, which is already bound to the first antibody. At the end of the reaction unreacted-labelled antibody is aspirated out and the solid phase washed with a wash solution. The radioactivity in the solid phase is directly proportional to the concentration of analyte present.
Why RIA technology?
RIA is a widely recognized laboratory method known for its sensitivity and specificity in detecting various analytes, whether biomarkers, hormones, antibodies, or other substances. Its use enables precise and reliable results, making it a valuable tool in biomedical research, clinical diagnostics, and other fields.
A major advantage of RIA is that it combines specificity of immunoassays with the sensitivity of radiochemical methods. Concentrations in the picomolar region can be measured accurately. This is obviously of great significance in monitoring or determining blood levels of certain hormones or therapeutic agents that seldom exceed a few micrograms per millilitre. When a higher level of sensitivity is needed, radioactivity is used for detection rather than colour (as is the case for ELISA). RIA used to be carried out in tubes rather than in microtiter plates to facilitate radioactive measurements in a scintillation counter. Moreover, RIA do not use enzymes and thus reduces the risk of interference from the sample itself.
Who can benefit from RIA utilization?
RIA is used by various industries and companies, including research laboratories, medical centers, pharmaceutical and biotechnology companies, as well as academic institutions. Whether you are a researcher, clinician, or industry professional, leveraging RIA technology can be beneficial in addressing your specific needs.
Numerous public and private laboratories have already benefited from the use of our RIA technology worldwide. Whether for fundamental research studies, diagnostic tests, or drug evaluations, RIA has been a valuable tool for obtaining precise and reliable results.
Explore DiaSource's expertise in this field. We understand the crucial role of RIA (RadioImmunoAssay) technology across various sectors, and at DiaSource, we stand ready to accompany you and be your trusted partner in unlocking its full potential.
DiaSource comprehends the significance and nuances of RIA technology. We have cultivated substantial expertise in its utilization and effective application. As your partner, we are committed to providing you not only with the necessary products and services but also the guidance and support you need to successfully achieve your goals.
